basic pcat vector (Promega)
90
Structured Review
Promega
basic pcat vector
Basic Pcat Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+vector/pcat+basic/pmc11193803-334-39-41
Average 90 stars, based on 1 article reviews
Basic Pcat Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+vector/pcat+basic/pmc11193803-334-39-41
Average 90 stars, based on 1 article reviews
basic pcat vector - by Bioz Stars,
2026-09
90/100 stars
Images
Related Articles
Plasmid Preparation:Article Title: Synthesis, characterization and transfection activity of new saturated and unsaturated cationic lipids. Article Snippet: We synthesized new cationic lipids, analogue to N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) and 1,2-dimyristyloxypropyl-3-dimethyl-hydroxyethylammonium bromide (DMRIE), in order to compare those containing a dodecyl chain with those having a relatively long chain with two or five double bonds, such as squalenyl and dihydrofarnesyl derivatives, or complex saturated structures, such as squalane derivatives.. The fusogenic helper lipid dioleoylphosphatidylethanolamine (DOPE) was added to cationic lipids to form a stable complex.. Liposomes composed of 50:50 w/w cationic lipid/DOPE were prepared and incubated with plasmidic DNA at various charge ratios and the diameter and zeta potential of the complexes were measured. Article Title: Articular cartilage gene therapy with recombinant vector encoding BMP-7 Article Snippet: .. The purified HindIII/PstI 166 bp DNA fragment is cloned into the corresponding sites of the Article Title: Transcriptional activation of the steroidogenic acute regulatory protein (StAR) gene: GATA-4 and CCAAT/enhancer-binding protein beta confer synergistic responsiveness in hormone-treated rat granulosa and HEK293 cell models. Article Snippet: Steroidogenic acute regulatory protein (StAR) mediates translocation of cholesterol to the inner membranes of steroidogenic mitochondria, where it serves as a substrate for steroid synthesis.. Transcription of StAR in the gonads and adrenal cells is upregulated by trophic hormones, involves downstream signaling pathways and a cohort of trans-factors acting as activators or suppressors of StAR transcription.. This study suggests that a 21 basepair long sequence positioned at −81/−61 of the murine StAR promoter is sufficient to confer a robust hormonal activation of t b k t p o w S © Article Title: Increased Sp1 phosphorylation as a mechanism of hepatocyte growth factor (HGF/SF)-induced vascular endothelial growth factor (VEGF/VPF) transcription. Article Snippet: .. Briefly, the indicated promoter fragment with flanking 5′-HindIII and 3′-XhoI enzyme restriction sites to facilitate directional cloning into the Article Title: Regulation of heavy subunit chain of gamma-glutamylcysteine synthetase by tumor necrosis factor-alpha in lens epithelial cells: role of LEDGF/p75. Article Snippet: Takamura, Yoshihiro, Nigar Fatma, Eri Kubo, and Dhirendra P. Singh.. Regulation of heavy subunit chain of -glutamylcysteine synthetase by tumor necrosis factorin lens epithelial cells: role of LEDGF/p75.. Am J Physiol Cell Physiol 290: C554–C566, 2006; doi:10.1152/ajpcell.00398.2005.—TNFinduces oxidative stress by generating reactive oxygen species (ROS). Article Title: Shear Stress Regulates Endothelial Nitric-oxide Synthase Promoter Activity through Nuclear Factor κB Binding Article Snippet: Human eNOS Promoter Deletion and Mutagenesis—A modified pGL2 vector was used as a CAT reporter gene vector. .. This was generated by replacement of the luciferase gene of pGL2 Basic vector (Promega, Madison, WI) by the CAT gene derived from Article Title: Cyclic adenosine monophosphate (cAMP) stimulation of the kit ligand promoter in sertoli cells requires an Sp1-binding region, a canonical TATA box, and a cAMP-induced factor binding to an immediately downstream GC-rich element. Article Snippet: .. Polymerase chain reaction (PCR) product was cloned into SalI/XbaI sites of Article Title: HOXA10 Controls Osteoblastogenesis by Directly Activating Bone Regulatory and Phenotypic Genes Article Snippet: All results were normalized to the luciferase activity resulting from transfection of the promoterless pGL3 luciferase construct (Promega, Madison, WI). .. The OC promoter activity was assayed by using the 208-bp promoter DNA fragment from rat OC genes cloned in the Luciferase:Article Title: Synthesis, characterization and transfection activity of new saturated and unsaturated cationic lipids. Article Snippet: We synthesized new cationic lipids, analogue to N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) and 1,2-dimyristyloxypropyl-3-dimethyl-hydroxyethylammonium bromide (DMRIE), in order to compare those containing a dodecyl chain with those having a relatively long chain with two or five double bonds, such as squalenyl and dihydrofarnesyl derivatives, or complex saturated structures, such as squalane derivatives.. The fusogenic helper lipid dioleoylphosphatidylethanolamine (DOPE) was added to cationic lipids to form a stable complex.. Liposomes composed of 50:50 w/w cationic lipid/DOPE were prepared and incubated with plasmidic DNA at various charge ratios and the diameter and zeta potential of the complexes were measured. Article Title: Shear Stress Regulates Endothelial Nitric-oxide Synthase Promoter Activity through Nuclear Factor κB Binding Article Snippet: Human eNOS Promoter Deletion and Mutagenesis—A modified pGL2 vector was used as a CAT reporter gene vector. .. This was generated by replacement of the luciferase gene of pGL2 Basic vector (Promega, Madison, WI) by the CAT gene derived from Synthesized:Article Title: Synthesis, characterization and transfection activity of new saturated and unsaturated cationic lipids. Article Snippet: We synthesized new cationic lipids, analogue to N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) and 1,2-dimyristyloxypropyl-3-dimethyl-hydroxyethylammonium bromide (DMRIE), in order to compare those containing a dodecyl chain with those having a relatively long chain with two or five double bonds, such as squalenyl and dihydrofarnesyl derivatives, or complex saturated structures, such as squalane derivatives.. The fusogenic helper lipid dioleoylphosphatidylethanolamine (DOPE) was added to cationic lipids to form a stable complex.. Liposomes composed of 50:50 w/w cationic lipid/DOPE were prepared and incubated with plasmidic DNA at various charge ratios and the diameter and zeta potential of the complexes were measured. Article Title: Increased Sp1 phosphorylation as a mechanism of hepatocyte growth factor (HGF/SF)-induced vascular endothelial growth factor (VEGF/VPF) transcription. Article Snippet: .. Briefly, the indicated promoter fragment with flanking 5′-HindIII and 3′-XhoI enzyme restriction sites to facilitate directional cloning into the Purification:Article Title: Articular cartilage gene therapy with recombinant vector encoding BMP-7 Article Snippet: .. The purified HindIII/PstI 166 bp DNA fragment is cloned into the corresponding sites of the Clone Assay:Article Title: Articular cartilage gene therapy with recombinant vector encoding BMP-7 Article Snippet: .. The purified HindIII/PstI 166 bp DNA fragment is cloned into the corresponding sites of the Article Title: Cyclic adenosine monophosphate (cAMP) stimulation of the kit ligand promoter in sertoli cells requires an Sp1-binding region, a canonical TATA box, and a cAMP-induced factor binding to an immediately downstream GC-rich element. Article Snippet: .. Polymerase chain reaction (PCR) product was cloned into SalI/XbaI sites of Article Title: HOXA10 Controls Osteoblastogenesis by Directly Activating Bone Regulatory and Phenotypic Genes Article Snippet: All results were normalized to the luciferase activity resulting from transfection of the promoterless pGL3 luciferase construct (Promega, Madison, WI). .. The OC promoter activity was assayed by using the 208-bp promoter DNA fragment from rat OC genes cloned in the Cloning:Article Title: Increased Sp1 phosphorylation as a mechanism of hepatocyte growth factor (HGF/SF)-induced vascular endothelial growth factor (VEGF/VPF) transcription. Article Snippet: .. Briefly, the indicated promoter fragment with flanking 5′-HindIII and 3′-XhoI enzyme restriction sites to facilitate directional cloning into the Polymerase Chain Reaction:Article Title: Increased Sp1 phosphorylation as a mechanism of hepatocyte growth factor (HGF/SF)-induced vascular endothelial growth factor (VEGF/VPF) transcription. Article Snippet: .. Briefly, the indicated promoter fragment with flanking 5′-HindIII and 3′-XhoI enzyme restriction sites to facilitate directional cloning into the Article Title: Cyclic adenosine monophosphate (cAMP) stimulation of the kit ligand promoter in sertoli cells requires an Sp1-binding region, a canonical TATA box, and a cAMP-induced factor binding to an immediately downstream GC-rich element. Article Snippet: .. Polymerase chain reaction (PCR) product was cloned into SalI/XbaI sites of Generated:Article Title: Regulation of heavy subunit chain of gamma-glutamylcysteine synthetase by tumor necrosis factor-alpha in lens epithelial cells: role of LEDGF/p75. Article Snippet: Takamura, Yoshihiro, Nigar Fatma, Eri Kubo, and Dhirendra P. Singh.. Regulation of heavy subunit chain of -glutamylcysteine synthetase by tumor necrosis factorin lens epithelial cells: role of LEDGF/p75.. Am J Physiol Cell Physiol 290: C554–C566, 2006; doi:10.1152/ajpcell.00398.2005.—TNFinduces oxidative stress by generating reactive oxygen species (ROS). Article Title: Shear Stress Regulates Endothelial Nitric-oxide Synthase Promoter Activity through Nuclear Factor κB Binding Article Snippet: Human eNOS Promoter Deletion and Mutagenesis—A modified pGL2 vector was used as a CAT reporter gene vector. .. This was generated by replacement of the luciferase gene of pGL2 Basic vector (Promega, Madison, WI) by the CAT gene derived from Amplification:Article Title: Regulation of heavy subunit chain of gamma-glutamylcysteine synthetase by tumor necrosis factor-alpha in lens epithelial cells: role of LEDGF/p75. Article Snippet: Takamura, Yoshihiro, Nigar Fatma, Eri Kubo, and Dhirendra P. Singh.. Regulation of heavy subunit chain of -glutamylcysteine synthetase by tumor necrosis factorin lens epithelial cells: role of LEDGF/p75.. Am J Physiol Cell Physiol 290: C554–C566, 2006; doi:10.1152/ajpcell.00398.2005.—TNFinduces oxidative stress by generating reactive oxygen species (ROS). Derivative Assay:Article Title: Shear Stress Regulates Endothelial Nitric-oxide Synthase Promoter Activity through Nuclear Factor κB Binding Article Snippet: Human eNOS Promoter Deletion and Mutagenesis—A modified pGL2 vector was used as a CAT reporter gene vector. .. This was generated by replacement of the luciferase gene of pGL2 Basic vector (Promega, Madison, WI) by the CAT gene derived from Activity Assay:Article Title: HOXA10 Controls Osteoblastogenesis by Directly Activating Bone Regulatory and Phenotypic Genes Article Snippet: All results were normalized to the luciferase activity resulting from transfection of the promoterless pGL3 luciferase construct (Promega, Madison, WI). .. The OC promoter activity was assayed by using the 208-bp promoter DNA fragment from rat OC genes cloned in the |
